permeabilisation buffer Search Results


permeabilization buffer  (TaKaRa)
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TaKaRa permeabilization buffer
Permeabilization Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facs permeabilising buffer  (Becton Dickinson)
90
Becton Dickinson facs permeabilising buffer
Facs Permeabilising Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facstm permeabilisation buffer  (Becton Dickinson)
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Becton Dickinson facstm permeabilisation buffer
Facstm Permeabilisation Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosflowtm fix and permeabilisation buffer ii  (Becton Dickinson)
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Becton Dickinson phosflowtm fix and permeabilisation buffer ii
Phosflowtm Fix And Permeabilisation Buffer Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeabilising blocking buffer containing 0.05% thimerosal fluka 71230  (Fluka Chemical)
90
Fluka Chemical permeabilising blocking buffer containing 0.05% thimerosal fluka 71230
Permeabilising Blocking Buffer Containing 0.05% Thimerosal Fluka 71230, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeabilisation buffer iii  (Becton Dickinson)
90
Becton Dickinson permeabilisation buffer iii
Permeabilisation Buffer Iii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytoperm permeabilisation buffer plus  (Becton Dickinson)
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Becton Dickinson cytoperm permeabilisation buffer plus
Cytoperm Permeabilisation Buffer Plus, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1× permeabilising wash buffer  (Becton Dickinson)
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Becton Dickinson 1× permeabilising wash buffer
1× Permeabilising Wash Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeabilisation wash buffer  (Becton Dickinson)
90
Becton Dickinson permeabilisation wash buffer
Permeabilisation Wash Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facs tm permeabilisation buffer  (Becton Dickinson)
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Becton Dickinson facs tm permeabilisation buffer
(A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8 + T-cell subsets. The percentage and mean fluorescence intensity for the CD57 + cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown ( n = 24). (B) Expression of CD57 on CD8 + T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection. (C) CD69 expression and CFSE proliferation profile for CD8 + T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown. (D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8 + T-cell subsets <t>FACS-sorted</t> on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown. (E) CD57 and perforin expression in the CD8 + T-cell population dissected into naïve (CD27 +high , perforin-negative), antigen-experienced CD27 + (perforin low ), and antigen-experienced CD27 − perforin low or perforin high subsets. The percentage and mean fluorescence intensity for the CD57 + cells are indicated. (F) Representative staining for perforin and CD57 in CD8 + T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown. (G) Representative staining for perforin and CD57 in CD4 + T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.
Facs Tm Permeabilisation Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeabilisation buffer solution  (Becton Dickinson)
90
Becton Dickinson permeabilisation buffer solution
(A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8 + T-cell subsets. The percentage and mean fluorescence intensity for the CD57 + cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown ( n = 24). (B) Expression of CD57 on CD8 + T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection. (C) CD69 expression and CFSE proliferation profile for CD8 + T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown. (D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8 + T-cell subsets <t>FACS-sorted</t> on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown. (E) CD57 and perforin expression in the CD8 + T-cell population dissected into naïve (CD27 +high , perforin-negative), antigen-experienced CD27 + (perforin low ), and antigen-experienced CD27 − perforin low or perforin high subsets. The percentage and mean fluorescence intensity for the CD57 + cells are indicated. (F) Representative staining for perforin and CD57 in CD8 + T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown. (G) Representative staining for perforin and CD57 in CD4 + T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.
Permeabilisation Buffer Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intracellular fixation permeabilisation buffer kit  (Becton Dickinson)
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Becton Dickinson intracellular fixation permeabilisation buffer kit
(A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8 + T-cell subsets. The percentage and mean fluorescence intensity for the CD57 + cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown ( n = 24). (B) Expression of CD57 on CD8 + T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection. (C) CD69 expression and CFSE proliferation profile for CD8 + T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown. (D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8 + T-cell subsets <t>FACS-sorted</t> on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown. (E) CD57 and perforin expression in the CD8 + T-cell population dissected into naïve (CD27 +high , perforin-negative), antigen-experienced CD27 + (perforin low ), and antigen-experienced CD27 − perforin low or perforin high subsets. The percentage and mean fluorescence intensity for the CD57 + cells are indicated. (F) Representative staining for perforin and CD57 in CD8 + T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown. (G) Representative staining for perforin and CD57 in CD4 + T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.
Intracellular Fixation Permeabilisation Buffer Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8 + T-cell subsets. The percentage and mean fluorescence intensity for the CD57 + cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown ( n = 24). (B) Expression of CD57 on CD8 + T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection. (C) CD69 expression and CFSE proliferation profile for CD8 + T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown. (D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8 + T-cell subsets FACS-sorted on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown. (E) CD57 and perforin expression in the CD8 + T-cell population dissected into naïve (CD27 +high , perforin-negative), antigen-experienced CD27 + (perforin low ), and antigen-experienced CD27 − perforin low or perforin high subsets. The percentage and mean fluorescence intensity for the CD57 + cells are indicated. (F) Representative staining for perforin and CD57 in CD8 + T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown. (G) Representative staining for perforin and CD57 in CD4 + T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.

Journal: PLoS Biology

Article Title: Immune Activation and CD8 + T-Cell Differentiation towards Senescence in HIV-1 Infection

doi: 10.1371/journal.pbio.0020020

Figure Lengend Snippet: (A) Expression of the replicative senescence-associated marker CD57 on antigen-experienced CD8 + T-cell subsets. The percentage and mean fluorescence intensity for the CD57 + cells are shown for one single donor. Data on several donors (HIV-1-infected or healthy) are also shown ( n = 24). (B) Expression of CD57 on CD8 + T-cells (whole population or antigen-specific) from acute to postacute (on ART) HIV-1 infection. (C) CD69 expression and CFSE proliferation profile for CD8 + T-cell subsets gated on the basis of CD57 and CD27 expression following stimulation with anti-CD3 antibodies. PBMCs were analysed for CD69 expression after 18 h and CFSE labeling after 6 d. Percentages of proliferating cells (with background subtracted) are indicated. Representative results from three experiments (one HIV-infected and two healthy donors) are shown. (D) Telomere length measurement by flow FISH on naïve and antigen-experienced CD8 + T-cell subsets FACS-sorted on the basis of CD57, CD27, CCR7, and CD45RA expression. The average length of telomeres was obtained by substracting the mean fluorescence of the background control (no probe; open histogram) from the mean fluorescence obtained from cells hybridised with the FITC-labeled telomere probe (gray histogram). Representative results from two experiments (on healthy donors) are shown. (E) CD57 and perforin expression in the CD8 + T-cell population dissected into naïve (CD27 +high , perforin-negative), antigen-experienced CD27 + (perforin low ), and antigen-experienced CD27 − perforin low or perforin high subsets. The percentage and mean fluorescence intensity for the CD57 + cells are indicated. (F) Representative staining for perforin and CD57 in CD8 + T-cells from a HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown. (G) Representative staining for perforin and CD57 in CD4 + T-cells from an HIV-1-infected or a healthy donor. Percentages of cells present in the top quadrants are shown.

Article Snippet: Cells were washed, fixed, and permeabilised in FACS TM permeabilisation buffer (Becton-Dickinson).

Techniques: Expressing, Marker, Fluorescence, Infection, Labeling, Control, Staining